%0 Figure %A A. Okoroma, Emeka %A Purchase, Diane %A Garelick, Hemda %A Morris, Roger %A H. Neale, Michael %A Windl, Otto %A O. Abiola, Oduola %D 2013 %T Western blot profile of ME7 brain homogenate digested with EF with and without BS. %U https://plos.figshare.com/articles/figure/_Western_blot_profile_of_ME7_brain_homogenate_digested_with_EF_with_and_without_BS_/746361 %R 10.1371/journal.pone.0068099.g001 %2 https://mdx.figshare.com/ndownloader/files/1120045 %K Biochemistry %K enzymes %K biotechnology %K Environmental biotechnology %K biodegradation %K bioremediation %K Applied microbiology %K Infectious diseases %K Prion diseases %K Veterinary diseases %K Zoonotic diseases %K Veterinary prion diseases %K blot %K me7 %K homogenate %K digested %K ef %X

Lanes 2, 3, 4, and 5 are ME7 scrapie brain homogenate digested at 65°C for 1 h with PK (100 µg/ml), EF+BS, BS and EF respectively. Total removal of PrPSc signal was achieved with EF+BS only. PrPSc was probed with SAF83 mAb.

%I PLOS ONE