%0 Figure %A A. Okoroma, Emeka %A Purchase, Diane %A Garelick, Hemda %A Morris, Roger %A H. Neale, Michael %A Windl, Otto %A O. Abiola, Oduola %D 2013 %T Time-course profile of ME7 brain homogenate digested with EF+BS at 65°C. %U https://plos.figshare.com/articles/figure/_Time_course_profile_of_ME7_brain_homogenate_digested_with_EF_BS_at_65_176_C_/746364 %R 10.1371/journal.pone.0068099.g003 %2 https://mdx.figshare.com/ndownloader/files/1120048 %K Biochemistry %K enzymes %K biotechnology %K Environmental biotechnology %K biodegradation %K bioremediation %K Applied microbiology %K Infectious diseases %K Prion diseases %K Veterinary diseases %K Zoonotic diseases %K Veterinary prion diseases %K me7 %K homogenate %K digested %X

Lane 1 is neat ME7 brain homogenate (positive control) and lane 2 is proteinase K digested sample (77 µg/ml final PK conc.). Lanes 3–6 are digested with EF+BS at 10, 30, 45 and 60 min. Samples were digested at 65°C and PrPSc was probed with SAF83 mAb.

%I PLOS ONE