TY - DATA T1 - The effect of inhibitors of active cellular transport on the localization of glucose-coated nanoparticles in hCMEC/D3 cells. PY - 2013/12/05 AU - Radka Gromnicova AU - Heather A. Davies AU - Peddagangannagari Sreekanthreddy AU - Ignacio A. Romero AU - Torben Lund AU - Ivan M. Roitt AU - James B. Phillips AU - David K. Male UR - https://plos.figshare.com/articles/figure/_The_effect_of_inhibitors_of_active_cellular_transport_on_the_localization_of_glucose_coated_nanoparticles_in_hCMEC_D3_cells_/869875 DO - 10.1371/journal.pone.0081043.g003 L4 - https://mdx.figshare.com/ndownloader/files/1305213 KW - inhibitors KW - cellular KW - localization KW - glucose-coated KW - nanoparticles N2 - (a) Cells were treated with 10 µg/ml nystatin, 5 µg/ml cytochalasin-D, 5 µg/ml nocodazole, 10 µg/ml chlorpromazine, 5 µg/ml cytochalasin-B. Data are expressed as the number of nanoparticles located below the basal membrane compared with untreated cells. Values are the mean ± SEM of at least 50 cells. Anova indicates no significant difference between treatments. (b) Localization of nanoparticles in hCMEC/D3 cells at 8 hours after application following incubation at 37°C or 30°C. U.M. = upper (apical) membrane, Cyt. = cytoplasmic, Ves. = vesicular, L.M. = lower (basal) membrane. The values are the mean ± SEM from at least 50 TEM images from a representative experiment. Data was analysed by Anova; there was no significant difference between the control and antibiotic treated samples (P = 0.703). ER -