Representative photograph of ELISPOT plate.

<p>The wells contained infected RK13<sup>VRQ.G9</sup> cells inoculated with IBH (SSBP/1), incubated IBH (heat treated SSBP/1) and BS (Biosurfactant digested SSBP/1). Wells inoculated with SSBP/1 digested with EF and EF+BS were completely devoid of infected cells. PrP<sup>Sc</sup> was probed with sha31 mAb.</p>