Time-course profile of ME7 brain homogenate digested with EF+BS at 50°C.

<p>Lane 1 is neat ME7 brain homogenate (positive control) and lane 2 is proteinase K treated sample (77 µg/ml final PK conc.). Lanes 3–7 are digested with EF+BS at 30–120 min respectively. Samples were digested at 50°C and PrP<sup>Sc</sup> was probed with SAF83 mAb.</p>