Western blot profile of ME7 brain homogenate digested with EF with and without BS.

<p>Lanes 2, 3, 4, and 5 are ME7 scrapie brain homogenate digested at 65°C for 1 h with PK (100 µg/ml), EF+BS, BS and EF respectively. Total removal of PrP<sup>Sc</sup> signal was achieved with EF+BS only. PrP<sup>Sc</sup> was probed with SAF83 mAb.</p>